Journal: Journal of Pain Research
Article Title: Electroacupuncture and Parecoxib Reduce Inflammatory Injury in a Primary Dysmenorrhea Rat Model: Investigating the Role of the COX-2/NF-κB/NLRP3 Pathway
doi: 10.2147/JPR.S512243
Figure Lengend Snippet: Immunofluorescence analysis of NF-κBp65 expression in rat uterine tissues. Fluorescence analysis software (AIPathwell), which uses AI technology, was used to identify localized cells (blue nuclei) in whole tissue sections to count the total cell number. The AI software identified red fluorescence in the whole tissue section as a positive signal for NF-κBp65 expression. When NF-κBp65 was expressed only in the cytoplasm, a red fluorescence signal was detected around the blue nucleus. When NF-κBp65 is also expressed in the nucleus, the red fluorescent signal in the nucleus overlaps with the blue fluorescence, leading to a purplish-red fluorescent signal. NF-κBp65 nuclear entry rate = nucleus positive cell count/(nucleus positive cell count + cytoplasm positive cell count). ( A ) Immunofluorescence images of NF-κBp65 expression in different groups; scale bar = 200 μm. ( B ) Bar chart of the NF-κBp65 nuclear entry rate (%) for each group. Data are means ± SD (n = 3).** P < 0.01, compared with the Saline group; ## P < 0.01, compared with the Model group; + P < 0.05, compared with the Ibuprofen group.
Article Snippet: The following instruments were used in this study: pathological tissue embedding instrument (BM-type, Anhui Research Institute of Electronic Science, Anhui, China); micro camera (Model 20486, Olympus, Tokyo, Japan); direct fluorescence microscope (DM4 B, Leica, Berlin, Germany); high-speed frozen centrifuge (Sorvall ST 16R, Thermo Fisher Scientific, Waltham, MA, USA); electrophoresis apparatus, transmembrane instrument, and gel imaging system (Gel Doc XR, Bio-Rad Laboratories, Hercules, CA, USA); ultrathin slicer EM UC7, Leica, Wetzlar, Germany) and diamond slicing knife (Ultra 45, Diatome AG, Nidau, Switzerland); panoramic slice scanner (3DHISTECH, Budapest, Hungary); ultramicrotomy (HT7700, HITACHI, Tokyo, Japan); electric acupuncture apparatus (SDZ-V type, Suzhou Medical Supplies Factory Co., Ltd., Suzhou, China); AIPathwell automated image analysis software (Servicebio Co, Ltd, Wuhan China).
Techniques: Immunofluorescence, Expressing, Fluorescence, Software, Cell Counting, Saline
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